Journal: Cell metabolism

Article Title: Abrogating mitochondrial dynamics in mouse hearts accelerates mitochondrial senescence

doi: 10.1016/j.cmet.2017.09.023

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyconal anti-LC3 A/B , Cell Signaling Technology , Cat# 4108.

Techniques: Virus, Plasmid Preparation, Recombinant, TUNEL Assay, Software

Journal: Environmental Science and Pollution Research International

Article Title: The impact of organic extracts of seasonal PM 2.5 on primary human lung epithelial cells and their chemical characterization

doi: 10.1007/s11356-021-14850-1

Figure Lengend Snippet: The effects of PM 2.5 on the expression of aging and macroautophagy markers in BEAS-2B cells. Cells were exposed to V.C. or PM 2.5 organic compounds (1%) for 24 h. Total cell lysates were extracted and then subjected to western blot analysis for p16, p21, p27, LC3B, and GAPDH. Densitometry analysis was performed using Scion image software

Article Snippet: Rabbit polyclonal anti-phospho-p44/42 MAPK (Thr202/Tyr204) (p-ERK), anti-ERK, and anti-light chain 3B (LC3B) antibodies, and U0126 (a highly selective inhibitor of MEK1 and MEK2) were obtained from Cell Signaling (Danvers, MA, USA).

Techniques: Expressing, Western Blot, Software

Journal: Environmental Science and Pollution Research International

Article Title: The impact of organic extracts of seasonal PM 2.5 on primary human lung epithelial cells and their chemical characterization

doi: 10.1007/s11356-021-14850-1

Figure Lengend Snippet: The effects of PM 2.5 on the expression of aging and autophagy markers in primary HAECs. Primary HAECs (n = 6) were exposed to V.C. or PM 2.5 organic compounds (1%) for 24 h. Total cell lysates were extracted and then subjected to Western blot analysis for p16, p21, p27, LC3B, and GAPDH ( a ). Densitometry analysis using Scion image software ( b ). Data are presented as the mean ± SE. **p < 0.05

Article Snippet: Rabbit polyclonal anti-phospho-p44/42 MAPK (Thr202/Tyr204) (p-ERK), anti-ERK, and anti-light chain 3B (LC3B) antibodies, and U0126 (a highly selective inhibitor of MEK1 and MEK2) were obtained from Cell Signaling (Danvers, MA, USA).

Techniques: Expressing, Western Blot, Software

Journal: Evidence-based complementary and alternative medicine : eCAM

Article Title: Tetrandrine-Induced Autophagy in MDA-MB-231 Triple-Negative Breast Cancer Cell through the Inhibition of PI3K/AKT/mTOR Signaling.

doi: 10.1155/2019/7517431

Figure Lengend Snippet: Figure 4: Comparison of p62/SQSTM1, Beclin1, and LC3 in MDA-MB-231 cells intervened with different concentrations of tetrandrine.

Article Snippet: Rabbit anti-LC3 Polyclonal Antibody (Cat. No: 12135-1-AP), Rabbit anti-p62/SQSTM1 Polyclonal Antibody (Cat. No: 18420-1-AP), Rabbit anti-Beclin1 Polyclonal Antibody (Cat. No: 11306-1-AP), Mouse anti-PTEN Monoclonal Antibody (Cat. No: 60300-1-Ig),Mouse anti-PI3K p85 (alpha) Monoclonal Antibody (Cat. No: 60225-1-Ig), Rabbit antiPI3K p110 (alpha) Polyclonal Antibody (Cat. No: 21890- 1-AP), Mouse anti-AKT Monoclonal Antibody (60203-2- Ig), and Mouse anti-beta Actin Monoclonal antibody (Cat. No: 66009-1-Ig) were purchased from Proteintech Group, Inc. (Chicago, USA).

Techniques: Comparison

Journal: BMC Nephrology

Article Title: KLHL3-dependent WNK4 degradation affected by potassium through the neddylation and autophagy pathway

doi: 10.1186/s12882-023-03257-4

Figure Lengend Snippet: The neddylation and autophagy in the KLHL3-dependent WNK4 degradation. a Immunoblotting was performed with antibodies against WNK4, pWNK4, KLHL3-myc, NEDD8 ,and cullin3. Compared with control groups, the expression of WNK and pWNK4 increases significantly in the experimental groups. Densitometry analysis of WNK4, pWNK4, KLHL3, Nedd8 and Cullin3 . ** P < 0.01, * P < 0.05 n = 6 b HEK293T cells were treated with an autophagy inhibitor (3MA) and MLN4924 separately and in combination for 24 h. The combined effect of 3MA and MLN4924 for WNK is stronger than the single effect. Densitometry analysis of WNK4, pWNK4, KLHL3, NEDD8 ,and LC3B.**** P <0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05 n = 4 c Another autophagy inhibitor, Bafilomycin A1, is similar to 3MA

Article Snippet: Immunoblots were probed using the following primary antibodies: anti cullin3 antibody (Cell Signaling Technology), anti-β-actin monoclonal antibody (Santa Cruz), anti-NEDD8 antibody (Cell Signaling Technology), anti-c-Myc antibody (Sigma-Aldrich), anti-p62 antibody (MBL), anti-light chain 3B antibody (LC3B; MBL and Cell Signaling Technology), anti-glyceraldehyde-3-phosphate dehydrogenase antibody (GAPDH; Cell Signaling Technology), anti WNK4 and anti pWNK4 antibody (from Oregon Health & Science University), anti Keap1 antibody (Santa Cruz), anti Nrf2 antibody (Santa Cruz).

Techniques: Western Blot, Expressing

Journal: BMC Nephrology

Article Title: KLHL3-dependent WNK4 degradation affected by potassium through the neddylation and autophagy pathway

doi: 10.1186/s12882-023-03257-4

Figure Lengend Snippet: Potassium regulates WNK4 via autophagy and apoptosis. HEK293 cells co-expressed with KLHL3 and WNK4 kinase were treated by different potassium ion concentrations shown in a and b . It shows that the abundance of WNK4，pWNK4，NEDD8-Cullin3,capase3 ,and LC3B II increases in LK groups more than in HK groups. The expression of WNK4 and pWNK4 is higher with two drugs (MLN4924 and Bafilomycin A1) than MLN4924 only. Densitometry analysis of WNK4, pWNK4, KLHL3, NEDD8, CUL3, P62, KEAP1 and LC3B. **** P <0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05 n = 4

Article Snippet: Immunoblots were probed using the following primary antibodies: anti cullin3 antibody (Cell Signaling Technology), anti-β-actin monoclonal antibody (Santa Cruz), anti-NEDD8 antibody (Cell Signaling Technology), anti-c-Myc antibody (Sigma-Aldrich), anti-p62 antibody (MBL), anti-light chain 3B antibody (LC3B; MBL and Cell Signaling Technology), anti-glyceraldehyde-3-phosphate dehydrogenase antibody (GAPDH; Cell Signaling Technology), anti WNK4 and anti pWNK4 antibody (from Oregon Health & Science University), anti Keap1 antibody (Santa Cruz), anti Nrf2 antibody (Santa Cruz).

Techniques: Expressing

Journal: BMC Nephrology

Article Title: KLHL3-dependent WNK4 degradation affected by potassium through the neddylation and autophagy pathway

doi: 10.1186/s12882-023-03257-4

Figure Lengend Snippet: The effect of potassium on the CUL3-KLHL3-WNK4 cascade in vivo. Mice were fed with HK and LK diet and then were injected with MLN4924. Within LK groups or HK groups, the expression of pWNK4 and WNK4 increased with MLN4924 treatment. Densitometry analysis of WNK4, pWNK4, KLHL3, NEDD8, CUL3, P62, BECN1, KEAP1 and LC3B.**** P <0.0001, *** P < 0.001, ** P < 0.01, * P < 0.05 n =4

Article Snippet: Immunoblots were probed using the following primary antibodies: anti cullin3 antibody (Cell Signaling Technology), anti-β-actin monoclonal antibody (Santa Cruz), anti-NEDD8 antibody (Cell Signaling Technology), anti-c-Myc antibody (Sigma-Aldrich), anti-p62 antibody (MBL), anti-light chain 3B antibody (LC3B; MBL and Cell Signaling Technology), anti-glyceraldehyde-3-phosphate dehydrogenase antibody (GAPDH; Cell Signaling Technology), anti WNK4 and anti pWNK4 antibody (from Oregon Health & Science University), anti Keap1 antibody (Santa Cruz), anti Nrf2 antibody (Santa Cruz).

Techniques: In Vivo, Injection, Expressing

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: RAB23 facilitates clathrin-coated nascent vesicle formation at the plasma membrane and modulates cell signaling

doi: 10.1007/s00018-025-05694-w

Figure Lengend Snippet:

Article Snippet: Rabbit anti-LC3 A/B , Cell signaling technology , Cat#12741.

Techniques: Marker, Recombinant, Lysis, Blocking Assay, Protease Inhibitor, Transfection, Bicinchoninic Acid Protein Assay, Derivative Assay, Plasmid Preparation, Software, Imaging